African Swine Fever Virus Fluorescence PCR Detection Kit

The kit has extremely high sensitivity and is specifically designed for variant strains, vaccine strains, recombinant strains, and gene deleted strains. It is suitable for "precise elimination" in positive fields and helps with early diagnosis and reduction of economic losses.

┃ Product Introduction:

Suitable for the detection of African swine fever virus in samples.

┃ Product Features:

1.Reliable performance: high sensitivity and specificity validated in clinical

2.Easy screening: 60 mins to complete tests

3.Cost-saving: compatible for most mainstream real-time PCR instruments, no specific system required

4.Internal control: monitoring sampling, nucleic acid extraction, and amplification processes.

┃ Product Information:

· Specification: 50 reactions/kit.

· Storage condition: Store at -20℃.

· Expiry date: 12 months.

┃ Product Background:

African swine fever (ASF), first identified in Kenya in 1921, was initially confined to sub-Saharan Africa. This highly lethal viral disease of swine, caused by the African swine fever virus (ASFV), typically results in near-complete mortality. Genetic and geographic variations in ASFV strains have significantly influenced global spread patterns. Notably, genotype I of ASFV emerged in Europe from western Africa in 1960, while genotype II — linked to a more virulent strain — entered the region via eastern Africa in 2007, subsequently proliferating across Europe. The virus reached China through Russia in 2018, with the first official report by the World Organisation for Animal Health (OIE) on 3 August 2018 confirming an outbreak in Shenyang, Liaoning Province. Despite stringent Chinese governmental interventions, the genotype II strain rapidly disseminated to all 31 provinces within the country.

Clinically, ASF manifestation in domestic pigs varies based on viral virulence, infection route/dose, and host factors, broadly categorized into hyperacute, acute, subacute, and chronic forms. Acute ASF is typified by sudden-onset hyperthermia (40–42°C), lethargy, anorexia, and rapid mortality. Subacute cases, associated with moderately virulent isolates, mirror acute symptoms but with attenuated severity; pigs exhibit prolonged fever (lasting 7–20 days post-infection), 30–70% mortality, and exacerbated hemorrhagic and edematous lesions compared to the acute form. Chronic ASF manifests as cutaneous necrosis, arthritis, growth stunting, respiratory distress, and abortion, with survival periods extending beyond two weeks. Notably, warthogs remain asymptomatic carriers.

ASFV’s environmental resilience — enabled by its complex capsid and genome structure — facilitates prolonged survival in contaminated environments. Transmission to domestic pigs occurs via direct contact with infected animals, fomites, or contaminated vectors, emphasizing the virus’s multifaceted ecological niche.